human negative selection-based nk isolation kit rosettesep Search Results


nk isolation kit  (Miltenyi Biotec)
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Miltenyi Biotec nk isolation kit
Nk Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-cd3/cd28 beads  (Thermo Fisher)
90
Thermo Fisher anti-cd3/cd28 beads
Validation of ectopically expressed myeloid-specific suppressive genes uncovered by G + -specific transcriptome. (Α) IDO-1 protein expression in G + cells. Representative flow cytometry plots for intracellular IDO-1 detection (left) and collective data of IDO-1 Median Fluorescence Intensity (MdFI) in G + T-cells (blue) over G - T-cells (dark grey) (n=8, p=0.001) and PBS-treated controls (light grey) (n=5, p=0.014) (right). (B) Enzyme-Linked Immunosorbent Assay (ELISA) for quantitative analysis of the Tryptophan (Trp) catabolite Kynurenine (Kyn) concentrations (left) and Kyn to Trp ratio (Kyn/Trp) (right) depicting the enzymatic activity of IDO-1 by the means of increased Kyn and Kyn/Trp ratio in G + cell culture supernatants. (C) Luminex assay for quantitative assessment of chemokines in cell culture supernatants of G + , G - and PBS-treated control cells (n=4-5) at rest (left) or after <t>anti-CD3/CD28</t> stimulation (right) revealing the increased secretion of CCL17 and CCL22. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, and ns: not significant.
Anti Cd3/Cd28 Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rosettesep human t cell enrichment cocktail  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc rosettesep human t cell enrichment cocktail
Validation of ectopically expressed myeloid-specific suppressive genes uncovered by G + -specific transcriptome. (Α) IDO-1 protein expression in G + cells. Representative flow cytometry plots for intracellular IDO-1 detection (left) and collective data of IDO-1 Median Fluorescence Intensity (MdFI) in G + T-cells (blue) over G - T-cells (dark grey) (n=8, p=0.001) and PBS-treated controls (light grey) (n=5, p=0.014) (right). (B) Enzyme-Linked Immunosorbent Assay (ELISA) for quantitative analysis of the Tryptophan (Trp) catabolite Kynurenine (Kyn) concentrations (left) and Kyn to Trp ratio (Kyn/Trp) (right) depicting the enzymatic activity of IDO-1 by the means of increased Kyn and Kyn/Trp ratio in G + cell culture supernatants. (C) Luminex assay for quantitative assessment of chemokines in cell culture supernatants of G + , G - and PBS-treated control cells (n=4-5) at rest (left) or after <t>anti-CD3/CD28</t> stimulation (right) revealing the increased secretion of CCL17 and CCL22. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, and ns: not significant.
Rosettesep Human T Cell Enrichment Cocktail, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rosettesep human monocyte enrichment cocktail  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc rosettesep human monocyte enrichment cocktail
Validation of ectopically expressed myeloid-specific suppressive genes uncovered by G + -specific transcriptome. (Α) IDO-1 protein expression in G + cells. Representative flow cytometry plots for intracellular IDO-1 detection (left) and collective data of IDO-1 Median Fluorescence Intensity (MdFI) in G + T-cells (blue) over G - T-cells (dark grey) (n=8, p=0.001) and PBS-treated controls (light grey) (n=5, p=0.014) (right). (B) Enzyme-Linked Immunosorbent Assay (ELISA) for quantitative analysis of the Tryptophan (Trp) catabolite Kynurenine (Kyn) concentrations (left) and Kyn to Trp ratio (Kyn/Trp) (right) depicting the enzymatic activity of IDO-1 by the means of increased Kyn and Kyn/Trp ratio in G + cell culture supernatants. (C) Luminex assay for quantitative assessment of chemokines in cell culture supernatants of G + , G - and PBS-treated control cells (n=4-5) at rest (left) or after <t>anti-CD3/CD28</t> stimulation (right) revealing the increased secretion of CCL17 and CCL22. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, and ns: not significant.
Rosettesep Human Monocyte Enrichment Cocktail, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ficoll paque plus  (GE Healthcare)
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GE Healthcare ficoll paque plus
Validation of ectopically expressed myeloid-specific suppressive genes uncovered by G + -specific transcriptome. (Α) IDO-1 protein expression in G + cells. Representative flow cytometry plots for intracellular IDO-1 detection (left) and collective data of IDO-1 Median Fluorescence Intensity (MdFI) in G + T-cells (blue) over G - T-cells (dark grey) (n=8, p=0.001) and PBS-treated controls (light grey) (n=5, p=0.014) (right). (B) Enzyme-Linked Immunosorbent Assay (ELISA) for quantitative analysis of the Tryptophan (Trp) catabolite Kynurenine (Kyn) concentrations (left) and Kyn to Trp ratio (Kyn/Trp) (right) depicting the enzymatic activity of IDO-1 by the means of increased Kyn and Kyn/Trp ratio in G + cell culture supernatants. (C) Luminex assay for quantitative assessment of chemokines in cell culture supernatants of G + , G - and PBS-treated control cells (n=4-5) at rest (left) or after <t>anti-CD3/CD28</t> stimulation (right) revealing the increased secretion of CCL17 and CCL22. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, and ns: not significant.
Ficoll Paque Plus, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imagtm human naive cd8+ t-cell enrichment kit  (Becton Dickinson)
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Becton Dickinson imagtm human naive cd8+ t-cell enrichment kit
Validation of ectopically expressed myeloid-specific suppressive genes uncovered by G + -specific transcriptome. (Α) IDO-1 protein expression in G + cells. Representative flow cytometry plots for intracellular IDO-1 detection (left) and collective data of IDO-1 Median Fluorescence Intensity (MdFI) in G + T-cells (blue) over G - T-cells (dark grey) (n=8, p=0.001) and PBS-treated controls (light grey) (n=5, p=0.014) (right). (B) Enzyme-Linked Immunosorbent Assay (ELISA) for quantitative analysis of the Tryptophan (Trp) catabolite Kynurenine (Kyn) concentrations (left) and Kyn to Trp ratio (Kyn/Trp) (right) depicting the enzymatic activity of IDO-1 by the means of increased Kyn and Kyn/Trp ratio in G + cell culture supernatants. (C) Luminex assay for quantitative assessment of chemokines in cell culture supernatants of G + , G - and PBS-treated control cells (n=4-5) at rest (left) or after <t>anti-CD3/CD28</t> stimulation (right) revealing the increased secretion of CCL17 and CCL22. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, and ns: not significant.
Imagtm Human Naive Cd8+ T Cell Enrichment Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human recombinant il-2  (PeproTech)
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Validation of ectopically expressed myeloid-specific suppressive genes uncovered by G + -specific transcriptome. (Α) IDO-1 protein expression in G + cells. Representative flow cytometry plots for intracellular IDO-1 detection (left) and collective data of IDO-1 Median Fluorescence Intensity (MdFI) in G + T-cells (blue) over G - T-cells (dark grey) (n=8, p=0.001) and PBS-treated controls (light grey) (n=5, p=0.014) (right). (B) Enzyme-Linked Immunosorbent Assay (ELISA) for quantitative analysis of the Tryptophan (Trp) catabolite Kynurenine (Kyn) concentrations (left) and Kyn to Trp ratio (Kyn/Trp) (right) depicting the enzymatic activity of IDO-1 by the means of increased Kyn and Kyn/Trp ratio in G + cell culture supernatants. (C) Luminex assay for quantitative assessment of chemokines in cell culture supernatants of G + , G - and PBS-treated control cells (n=4-5) at rest (left) or after <t>anti-CD3/CD28</t> stimulation (right) revealing the increased secretion of CCL17 and CCL22. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, and ns: not significant.
Human Recombinant Il 2, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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magnetic cell selection  (Miltenyi Biotec)
99
Miltenyi Biotec magnetic cell selection
Validation of ectopically expressed myeloid-specific suppressive genes uncovered by G + -specific transcriptome. (Α) IDO-1 protein expression in G + cells. Representative flow cytometry plots for intracellular IDO-1 detection (left) and collective data of IDO-1 Median Fluorescence Intensity (MdFI) in G + T-cells (blue) over G - T-cells (dark grey) (n=8, p=0.001) and PBS-treated controls (light grey) (n=5, p=0.014) (right). (B) Enzyme-Linked Immunosorbent Assay (ELISA) for quantitative analysis of the Tryptophan (Trp) catabolite Kynurenine (Kyn) concentrations (left) and Kyn to Trp ratio (Kyn/Trp) (right) depicting the enzymatic activity of IDO-1 by the means of increased Kyn and Kyn/Trp ratio in G + cell culture supernatants. (C) Luminex assay for quantitative assessment of chemokines in cell culture supernatants of G + , G - and PBS-treated control cells (n=4-5) at rest (left) or after <t>anti-CD3/CD28</t> stimulation (right) revealing the increased secretion of CCL17 and CCL22. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, and ns: not significant.
Magnetic Cell Selection, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rosettesep human b cells  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc rosettesep human b cells
Validation of ectopically expressed myeloid-specific suppressive genes uncovered by G + -specific transcriptome. (Α) IDO-1 protein expression in G + cells. Representative flow cytometry plots for intracellular IDO-1 detection (left) and collective data of IDO-1 Median Fluorescence Intensity (MdFI) in G + T-cells (blue) over G - T-cells (dark grey) (n=8, p=0.001) and PBS-treated controls (light grey) (n=5, p=0.014) (right). (B) Enzyme-Linked Immunosorbent Assay (ELISA) for quantitative analysis of the Tryptophan (Trp) catabolite Kynurenine (Kyn) concentrations (left) and Kyn to Trp ratio (Kyn/Trp) (right) depicting the enzymatic activity of IDO-1 by the means of increased Kyn and Kyn/Trp ratio in G + cell culture supernatants. (C) Luminex assay for quantitative assessment of chemokines in cell culture supernatants of G + , G - and PBS-treated control cells (n=4-5) at rest (left) or after <t>anti-CD3/CD28</t> stimulation (right) revealing the increased secretion of CCL17 and CCL22. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, and ns: not significant.
Rosettesep Human B Cells, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Validation of ectopically expressed myeloid-specific suppressive genes uncovered by G + -specific transcriptome. (Α) IDO-1 protein expression in G + cells. Representative flow cytometry plots for intracellular IDO-1 detection (left) and collective data of IDO-1 Median Fluorescence Intensity (MdFI) in G + T-cells (blue) over G - T-cells (dark grey) (n=8, p=0.001) and PBS-treated controls (light grey) (n=5, p=0.014) (right). (B) Enzyme-Linked Immunosorbent Assay (ELISA) for quantitative analysis of the Tryptophan (Trp) catabolite Kynurenine (Kyn) concentrations (left) and Kyn to Trp ratio (Kyn/Trp) (right) depicting the enzymatic activity of IDO-1 by the means of increased Kyn and Kyn/Trp ratio in G + cell culture supernatants. (C) Luminex assay for quantitative assessment of chemokines in cell culture supernatants of G + , G - and PBS-treated control cells (n=4-5) at rest (left) or after anti-CD3/CD28 stimulation (right) revealing the increased secretion of CCL17 and CCL22. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, and ns: not significant.

Journal: Frontiers in Immunology

Article Title: Hypomethylation-induced regulatory programs in T cells unveiled by transcriptomic analyses

doi: 10.3389/fimmu.2023.1235661

Figure Lengend Snippet: Validation of ectopically expressed myeloid-specific suppressive genes uncovered by G + -specific transcriptome. (Α) IDO-1 protein expression in G + cells. Representative flow cytometry plots for intracellular IDO-1 detection (left) and collective data of IDO-1 Median Fluorescence Intensity (MdFI) in G + T-cells (blue) over G - T-cells (dark grey) (n=8, p=0.001) and PBS-treated controls (light grey) (n=5, p=0.014) (right). (B) Enzyme-Linked Immunosorbent Assay (ELISA) for quantitative analysis of the Tryptophan (Trp) catabolite Kynurenine (Kyn) concentrations (left) and Kyn to Trp ratio (Kyn/Trp) (right) depicting the enzymatic activity of IDO-1 by the means of increased Kyn and Kyn/Trp ratio in G + cell culture supernatants. (C) Luminex assay for quantitative assessment of chemokines in cell culture supernatants of G + , G - and PBS-treated control cells (n=4-5) at rest (left) or after anti-CD3/CD28 stimulation (right) revealing the increased secretion of CCL17 and CCL22. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, and ns: not significant.

Article Snippet: T cells were isolated by negative magnetic cell selection (MACS Pan-T Cell Isolation Kit II Human MiltenyiBiotec, Germany) except for the flow cytometry-based validation experiments where CD4 + -selected T cells were used as starting material (RosetteSep Human CD4+ T-cell enrichment cocktail, StemCell Technologies, Canada), then activated for 3 days in the presence of anti-CD3/CD28 beads (Dynabeads Human T-Activator CD3/CD28, Gibco) at a 1:1 bead:cell ratio and subsequently incubated in the presence of 50U/mL human recombinant IL-2 (rhIL-2 PeproTech or Miltenyi Biotec) with 5 μM or 7.5 μM Dec (Sigma-Aldrich, Germany) for three additional days.

Techniques: Expressing, Flow Cytometry, Fluorescence, Enzyme-linked Immunosorbent Assay, Activity Assay, Cell Culture, Luminex